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Three new TIRF microscopy objectives

Olympus has introduced three new high-performance TIRF objectives. The 150x ultra-magnification objective offers a numerical aperture (NA) of 1.45 for optimal magnification, resolution, light gathering ability and imaging performance, and the 60x and 100x oil immersion objectives have NAs of 1.49.

The 150x UAPON objective is optimized for single molecule TIRF applications. It delivers the high magnification and low background noise necessary for visualizing challenging specimens. The objective delivers crisp images from 340nm ultraviolet (UV) to near-infrared wavelengths and comes with a correction collar to help compensate for aberrations caused by coverglass thickness or variations in immersion media temperatures.

The 100x UAPON objective also is newly enhanced with a correction collar, and has been updated to include near-UV performance. The 60x APON provides the brightest high-resolution TIRF images available, thanks to its improved point spread function.

More info at



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