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Microscope illuminator for multicolor TIRF imaging

Olympus has released the cell^TIRF illuminator. The system has four motorized laser channels for simultaneous image capture and allows instant setting and confirmation of the precise TIRF angle. Each of the four individually controlled laser inputs is optimally focused and the angle of each can be adjusted so that all wavelengths have the same penetration depth. Calculated penetration depths can be preset for all four lasers and used with a single mouse click; the system will automatically adjust the angle of each laser to simultaneously capture TIRF from all four channels. In addition, one laser line can be adjusted for FRAP experiments, and users can easily switch to widefield view and then reestablish TIRF imaging in less than 500 ms.

More information here.



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