New fluorescent protein constructs for labeling organelles

Organelle Lights reagents from Molecular Probes (Invitrogen) now come in new colors for labeling lysosomes, mitochondria, endoplasmic reticulum (ER), and endosomes (Lyosomes-GFP, Mito-RFP, ER-RFP, and endosomes-RFP). The prepackaged fluorescent protein constructs are fused with signal peptides and come ready-to-use. The reagents consist of suspensions of baculovirus carrying an expression construct with a fluorescent protein fused to either a cellular protein or a localization peptide. To use them for imaging, add the virus suspension to your cells and incubate overnight. The reagents can be used with other stains in live or fixed cells and let you avoid potentially harmful cell treatments.

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